By the Peptevity Research Desk — vendor-neutral, evidence-graded. Scientific review: independent reviewer pending — how our review works.

Peptide Reconstitution Chart: Worked Concentration Tables

Direct answer

A peptide reconstitution chart is a lookup table of the reconstitution math: it cross-references a vial's peptide mass (in milligrams) against the volume of bacteriostatic water added (in milliliters) and shows the resulting concentration — peptide mg ÷ diluent mL = mg/mL — plus the laboratory volume measured on a U-100 insulin-style syringe, where 1 unit = 0.01 mL. The tables below cover common vial sizes (5, 10, 15 mg) against common diluent volumes (1, 2, 3 mL). Every value is pure arithmetic — a unit conversion, not a recommendation. The outputs are laboratory volumes and concentrations, not doses. This page is research and educational information, not medical advice, it lists no compound "dose" presets, and it has no purchase links. The compounds people reconstitute are, in nearly every case, not FDA-approved and are sold "research use only — not for human consumption." Peptevity sells nothing.

How to read this chart — laboratory volumes, not doses

A reconstitution chart is just the reconstitution math laid out as a grid. Two numbers fully define any reconstituted solution: how much peptide is in the vial (its labeled mass, in milligrams) and how much sterile diluent you add (in milliliters). Divide one by the other and you have the concentration. The chart simply pre-computes that division for the vial sizes and diluent volumes that come up most often, so a bench worker can read the answer instead of calculating it.

Every cell below is a unit conversion, nothing more. We deliberately do not pre-fill any "typical" mass for a named compound, because that would be a dosing suggestion dressed as a convenience — the same firewall the peptide calculator holds. The U-100 column needs one definition: an insulin-style U-100 syringe is graduated so that 100 units = 1 mL, i.e., 1 unit = 0.01 mL. We use that only as arithmetic for measuring a laboratory volume. The amount of peptide sitting in one such 0.01 mL unit is the concentration times 0.01 mL — a number we list so the conversion is transparent, never as an instruction to draw or administer anything. This page lives in our highest-scrutiny section, the reconstitution and storage how-to silo, and the rule there is fixed: we publish the chemistry and the math, and no human-dosing protocols.

The two formulas behind every table:

  • Concentration (mg/mL) = peptide mass (mg) ÷ diluent volume (mL).
  • Peptide mass in one U-100 unit (0.01 mL) = concentration (mg/mL) × 0.01.

Reconstitution chart — concentration (mg/mL)

This first table answers the most common question: given this vial and this much water, what is the concentration? Read the vial size down the left, the bacteriostatic-water volume across the top.

Vial size (peptide) + 1 mL water + 2 mL water + 3 mL water
5 mg 5.0 mg/mL 2.5 mg/mL 1.67 mg/mL
10 mg 10.0 mg/mL 5.0 mg/mL 3.33 mg/mL
15 mg 15.0 mg/mL 7.5 mg/mL 5.0 mg/mL

The pattern is the trade-off reconstitution always makes: more diluent lowers the concentration. The same 5 mg vial is 5.0 mg/mL in 1 mL but 1.67 mg/mL in 3 mL — a lower concentration means any given mass of peptide occupies a larger volume of liquid, which is sometimes easier to measure accurately at the bench.

Per-unit laboratory volume — how much peptide sits in one U-100 unit

This second table expresses each concentration as the mass of peptide contained in a single U-100 syringe unit (0.01 mL). It is the concentration column multiplied by 0.01 mL. These are laboratory volumes, not doses — they describe what 0.01 mL of each solution physically contains, so a bench worker can convert between volume and mass with whatever graduation their instrument uses.

Vial size (peptide) + 1 mL water + 2 mL water + 3 mL water
5 mg 0.050 mg (50 µg) / unit 0.025 mg (25 µg) / unit 0.0167 mg (16.7 µg) / unit
10 mg 0.100 mg (100 µg) / unit 0.050 mg (50 µg) / unit 0.0333 mg (33.3 µg) / unit
15 mg 0.150 mg (150 µg) / unit 0.075 mg (75 µg) / unit 0.050 mg (50 µg) / unit

To go the other direction — what volume contains a target mass? — divide the target mass by the concentration: volume (mL) = target mass (mg) ÷ concentration (mg/mL), then multiply by 100 for U-100 units. For example, in the 10 mg-in-2 mL solution (5.0 mg/mL), a 0.25 mg working aliquot occupies 0.25 ÷ 5.0 = 0.05 mL = 5 units. That 5-unit figure is a laboratory volume — not a dose. The interactive version of this arithmetic lives on the peptide calculator page; this chart is the static, no-JavaScript reference it falls back to.

Worked example — reading one cell end to end

Take the center of the chart: a 10 mg vial reconstituted in 2 mL of bacteriostatic water.

  1. Concentration = 10 mg ÷ 2 mL = 5.0 mg/mL. (Chart, table 1, row "10 mg," column "+ 2 mL.")
  2. Per microliter = 5.0 mg/mL ÷ 1,000 = 0.005 mg/µL (5 µg/µL), since 1 mL = 1,000 µL.
  3. Per U-100 unit = 5.0 mg/mL × 0.01 mL = 0.05 mg (50 µg) in one unit. (Chart, table 2, same cell.)
  4. Volume for a 0.1 mg target = 0.1 mg ÷ 5.0 mg/mL = 0.02 mL = 20 µL = 2 units — a laboratory volume, not a dose.

Every step is division or multiplication by a constant. Nothing in this worked example recommends, implies, or calculates any human dose; it converts between mass, volume, and concentration for a known solution.

Why the diluent in the chart is bacteriostatic water

The "water" column above is not generic water. The standard laboratory diluent for water-soluble peptides is bacteriostatic water — sterile water carrying 0.9% (9 mg/mL) benzyl alcohol as an antimicrobial preservative, which is what lets a multi-use vial resist microbial growth across repeated entries (DailyMed — Bacteriostatic Water label). That preservative is not inert: benzyl alcohol can promote aggregation of some peptides and proteins through interaction with nonpolar side chains, and the label warns the product is "not for use in neonates" because of benzyl alcohol's association with fatal "gasping syndrome" in newborns (Factors affecting peptide aggregation, 2017, PMC5665799; NEJM, 1982).

The chart also assumes the peptide is water-soluble, which is not universal. Solubility is set by the peptide's sequence — its balance of charged versus hydrophobic residues — and manufacturer guidance is that highly hydrophobic or neutral peptides may need a small amount of an organic solvent (DMSO, acetonitrile, an alcohol) or dilute acid/base before diluting out, with the explicit recommendation to test solubility on a small aliquot first (Bachem, peptide solubility technical note). For a peptide that does not fully dissolve in water, the aqueous concentrations in this chart simply do not apply. The diluent itself is covered in depth on the bacteriostatic water explainer.

Caveats on the numbers

Three caveats keep the chart honest. First, it assumes the labeled vial mass is accurate — the arithmetic is only as good as the stated milligrams, and research-grade material is sold without the assay guarantees of an approved drug. Second, it assumes complete dissolution: a cloudy or partly dissolved solution is not at the charted concentration, and the cue there is to revisit solubility, not to force it (PMC5665799). Third, reconstitution starts a stability clock — once in solution a peptide is less stable than the dry powder, with temperature, light, and time as the variables; the chart describes the concentration at preparation, not a shelf-life guarantee (PMC5665799). Handling after reconstitution is covered on how to store peptides.

Honest bottom line

A peptide reconstitution chart is the reconstitution equation printed as a grid: peptide milligrams over diluent milliliters gives concentration, and the concentration times 0.01 mL gives what sits in one U-100 unit. The numbers are well-defined arithmetic, and the surrounding facts — the benzyl-alcohol preservative, the solubility dependence, the post-reconstitution stability clock — are sourced to manufacturer technical notes, peer-reviewed formulation reviews, and FDA labeling. What the chart deliberately does not do is cross from math into use: it names no vendors, lists no compound "dose" presets, and labels every measured volume a laboratory volume, not a dose. The compounds people reconstitute are largely not FDA-approved, are sold "research use only — not for human consumption," and sit in a regulatory picture actively shifting through 2026 and into 2027. Take the chart for what it is — a research-math reference — and watch the dated status on the living 2026 regulatory tracker.

Frequently asked questions

What is a peptide reconstitution chart? It is a lookup table that cross-references a vial's peptide mass (mg) against the volume of bacteriostatic water added (mL) and pre-computes the resulting concentration (peptide mg ÷ diluent mL = mg/mL), often alongside the laboratory volume on a U-100 syringe. Every value is a unit conversion. It is research and educational information, not medical advice, and not an instruction to prepare anything for human use; its outputs are laboratory volumes and concentrations, not doses.

How do I calculate concentration from the chart? Divide the peptide mass in milligrams by the diluent volume in milliliters. A 10 mg vial in 2 mL of bacteriostatic water is 10 ÷ 2 = 5.0 mg/mL; the same vial in 3 mL is 10 ÷ 3 ≈ 3.33 mg/mL. More diluent always lowers the concentration (Bachem technical note).

What does the "per unit" column mean, and is it a dose? A U-100 insulin-style syringe is graduated so that 100 units equal 1 mL, meaning 1 unit = 0.01 mL. The per-unit column shows the mass of peptide contained in that 0.01 mL of a given solution (concentration × 0.01). It is a volume-to-mass conversion for the bench, presented as arithmetic only — never a dosing instruction. The chart recommends no amount.

How do I find the volume that holds a target mass? Divide the target mass by the concentration: volume (mL) = target mass (mg) ÷ concentration (mg/mL), then multiply by 100 for U-100 units. For a 5.0 mg/mL solution and a 0.25 mg target, that is 0.25 ÷ 5.0 = 0.05 mL = 5 units. This is a unit conversion for measuring a laboratory volume, not a dose calculation. The peptide calculator automates it.

Does the chart depend on the peptide being water-soluble? Yes. The aqueous concentrations assume the peptide fully dissolves in bacteriostatic water. Solubility is governed by the peptide's sequence; highly hydrophobic or neutral peptides may need a small amount of an organic solvent or dilute acid/base first, and manufacturer guidance is to test a small aliquot before committing the sample (Bachem). If the solution is cloudy or incomplete, the charted concentration does not apply.

Are charted research peptides legal or FDA-approved? The compounds usually involved (BPC-157, TB-500, GHK-Cu, MOTS-c, and others) are not FDA-approved for any use. In April 2026 the FDA removed a group of such peptides from its interim 503A Category 2 list because the supporting nominations were withdrawn — removal is not approval — and a PCAC meeting on July 23–24, 2026 is reviewing whether to add seven (BPC-157, TB-500, KPV, MOTS-c, DSIP, epitalon, Semax) to the 503A bulks list, with a second review group (including GHK-Cu and Melanotan II) expected before the end of February 2027 (Federal Register, docket FDA-2025-N-6895). Material is typically sold "research use only — not for human consumption." See the dated 2026 regulatory tracker.

How we graded this page

This is a procedural/laboratory reference, not an efficacy claim. The chart values are arithmetic; every chemistry, stability, and regulatory statement around them is tied to a primary or manufacturer/regulator source per our evidence-grading methodology and sourcing and citation policy. Peptevity carries no advertising, no affiliate links, and sells nothing — see our conflict-of-interest and funding statement, and our medical disclaimer and RUO statement.

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